Catalytic Properties of Botulinum Neurotoxin Subtypes A3 and A4
نویسندگان
چکیده
منابع مشابه
Botulinum Neurotoxin Subtype A4 Originating from Nontoxigenic Clostridium botulinum.
In this issue of Applied and Environmental Microbiology (AEM), we publish a paper by Marite Bradshaw, William H. Tepp, Regina C. M. Whitemarsh, Sabine Pellett, and Eric A. Johnson from the Department of Bacteriology, University of Wisconsin— Madison (UW—M), reporting on the purification and characterization of subtype A4 botulinum neurotoxin (1). The authors describe the purification of recombi...
متن کاملHolotoxin Activity of Botulinum Neurotoxin Subtype A4 Originating from a Nontoxigenic Clostridium botulinum Expression System.
Clostridium botulinum subtype A4 neurotoxin (BoNT/A4) is naturally expressed in the dual-toxin-producing C. botulinum strain 657Ba at 100× lower titers than BoNT/B. In this study, we describe purification of recombinant BoNT/A4 (rBoNT/A4) expressed in a nonsporulating and nontoxigenic C. botulinum expression host strain. The rBoNT/A4 copurified with nontoxic toxin complex components provided in...
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BACKGROUND Clostridium botulinum and related clostridial species express extremely potent neurotoxins known as botulinum neurotoxins (BoNTs) that cause long-lasting, potentially fatal intoxications in humans and other mammals. The amino acid variation within the BoNT is used to categorize the species into seven immunologically distinct BoNT serotypes (A-G) which are further divided into subtype...
متن کاملComparative Study of Immunological and Structural Properties of Two Recombinant Vaccine Candidates against Botulinum Neurotoxin Type E
Background: Recently, botulinum neurotoxin (BoNT)-derived recombinant proteins have been suggested as potential botulism vaccines. Here, with concentrating on BoNT type E (BoNT/E), we studied two of these binding domain-based recombinant proteins: a multivalent chimer protein, which is composed of BoNT serotypes A, B and E binding subdomains, and a monovalent recombinant protein, which contains...
متن کاملOn Botulinum Neurotoxin Variability
The rapidly growing number of botulinum neurotoxin sequences poses the problem of the possible evolutionary significance of the variability of these superpotent neurotoxins for toxin-producing Clostridium species. To progress in the understanding of this remarkable phenomenon, we suggest that researchers should (i) abandon an anthropocentric view of these neurotoxins as human botulism-causing a...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2009
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi801686b